Untangling the spatial microbial diversity within roots of endemic saxicolous shrub Daphne arbuscula
| Name: | Untangling the spatial microbial diversity within roots of endemic saxicolous shrub Daphne arbuscula |
|---|---|
| Researchers: |
Kolaříková (roz. Sýkorová) Zuzana (head – principal researcher) De La Cruz Carbajal Henry Jose (member of research team) Nicoletti Marascalchi Matheus (member of research team) Rydlová Jana (member of research team) Sudová Radka (member of research team) |
| Provider: | AV ČR |
| Number: | SAV-25-04 |
| Realization from: | 2025 |
| Realization to: | 2026 |
| Summary: | Daphne arbuscula represents flagship species of Carpathians endemics with narrow distribution to extreme rocky habitats within a single mountain range. Our previous research (with limited number of plant individuals analyzed at each site) revealed significantly different communities of rootassociated fungi within those shrubs in different sites. Site Šiance exhibited a higher heterogeneity of microclimatic habitats (“warmer” and “colder” sites in close vicinity), which corresponded with our findings of two distinct root mycobiome patterns. We aim to deeply investigate microclimatedriven spatial patterns of microorganisms within the Daphne population at this single sampling site while minimizing other environmental influences. We will combine traditional microscopic screening of mycorrhizae with the high-throughput sequencing of three specific rDNA barcoding regions. We will extend our understanding of D. arbuscula root microbial communities by analyzing the roots of the entire population from Šiance and by including an additional marker to cover underexplored bacterial microbiome. |