Integrating proteomic and genomic tools to contribute evolutionary processes across the plant kingdom with emphasis to the family of cytokinins

Name: Integrating proteomic and genomic tools to contribute evolutionary processes across the plant kingdom with emphasis to the family of cytokinins
Researchers: Záveská Drábková Lenka (co-researcher)
Project Type: B - national grants (GA ČR, GA AVČR)
Realization from: 2011
Realization to: 2014
Summary: Construction of phylogenetic trees is a powerful tool for understanding plant biology. Many different molecular markers have been used in phylogenetic studies to elucidate relationships within the plants. This project is aimed to the family of cytokinins (CKs), phytohormones that play an important role in plant growth and development, with emphasis to study their deactivation. The main objective consists in (1) detailed screening and defining distribution of CK deactivation forms (N- and O-glucosides, cis-zeatin [cisZ] derivatives) throughout evolution of plant kingdom, (2) including cisZ-O-glucosyltransferase (cisZOG1, UDP-glucosyltransferase family) gene as an integrating element of the studied CK down-regulatory pathways to the known phylogenetic signals (e.g. rbcL, atpB, matK, 18S rDNA, 26S rDNA) and performing its phylogeny in plants and (3) characterizing very multiple features of metabolic formation and conversions of CK glucosides as well as cisZ-type CKs, their regulations and role in physiologically important processes (plant stress responses, senescence, morphogenesis).

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