{"id":408,"date":"2019-06-14T12:06:50","date_gmt":"2019-06-14T10:06:50","guid":{"rendered":"https:\/\/www.ibot.cas.cz\/oddeleni-evolucni-biologie-rostlin\/?page_id=408"},"modified":"2024-12-09T13:34:40","modified_gmt":"2024-12-09T12:34:40","slug":"laborator-prutokove-cytometrie","status":"publish","type":"page","link":"https:\/\/www.ibot.cas.cz\/oddeleni-evolucni-biologie-rostlin\/?page_id=408&lang=cs","title":{"rendered":"Laborato\u0159 pr\u016ftokov\u00e9 cytometrie"},"content":{"rendered":"<p><img loading=\"lazy\" decoding=\"async\" class=\"wp-image-409 size-medium\" src=\"https:\/\/www.ibot.cas.cz\/oddeleni-evolucni-biologie-rostlin\/wp-content\/uploads\/sites\/7\/2019\/06\/PC204780-300x200.jpg\" alt=\"\" width=\"300\" height=\"200\" srcset=\"https:\/\/www.ibot.cas.cz\/oddeleni-evolucni-biologie-rostlin\/wp-content\/uploads\/sites\/7\/2019\/06\/PC204780-300x200.jpg 300w, https:\/\/www.ibot.cas.cz\/oddeleni-evolucni-biologie-rostlin\/wp-content\/uploads\/sites\/7\/2019\/06\/PC204780-768x512.jpg 768w, https:\/\/www.ibot.cas.cz\/oddeleni-evolucni-biologie-rostlin\/wp-content\/uploads\/sites\/7\/2019\/06\/PC204780-1024x683.jpg 1024w\" sizes=\"auto, (max-width: 300px) 100vw, 300px\" \/><\/p>\n<p>Laborato\u0159 pr\u016ftokov\u00e9 cytometrie byla zalo\u017eena v z\u00e1\u0159\u00ed 2000 a je nyn\u00ed sou\u010d\u00e1st\u00ed <a href=\"https:\/\/www.ibot.cas.cz\/cs\/labs\/oddeleni-evolucni-biologie-rostlin\/\">Odd\u011blen\u00ed evolu\u010dn\u00ed biologie rostlin.<\/a> Zab\u00fdv\u00e1 se rychl\u00fdm stanoven\u00edm obsahu jadern\u00e9 DNA (velikosti genomu) v relativn\u00edch i absolutn\u00edch jednotk\u00e1ch. Z\u00edskan\u00e9 \u00fadaje jsou vyu\u017e\u00edv\u00e1ny ke studiu diverzity a dynamiky polyploidn\u00edch komplex\u016f c\u00e9vnat\u00fdch rostlin \u010di ke zhodnocen\u00ed evolu\u010dn\u00edch a ekologick\u00fdch d\u016fsledk\u016f genomov\u00e9 duplikace (polyploidie) a variability v chromozomov\u00fdch po\u010dtech (aneuploidie). Dal\u0161\u00ed okruhy \u0159e\u0161en\u00fdch ot\u00e1zek zahrnuj\u00ed studium vlivu velikosti jadern\u00e9ho genomu na vlastnosti organism\u016f a stanoven\u00ed zp\u016fsob\u016f reprodukce. Nespornou v\u00fdhodou pr\u016ftokov\u00e9 cytometrie je zna\u010dn\u00e1 rychlost anal\u00fdz, kter\u00e1 umo\u017e\u0148uje analyzovat rozs\u00e1hl\u00e9 popula\u010dn\u00ed vzorky a z\u00edskat tak informace o cytotypov\u00e9 variabilit\u011b na r\u016fzn\u00fdch \u00farovn\u00edch organizace i na r\u016fzn\u00fdch prostorov\u00fdch a \u010dasov\u00fdch \u0161k\u00e1l\u00e1ch. Ve studi\u00edch je kladen d\u016fraz na propojen\u00ed cytometrick\u00fdch technik s dal\u0161\u00edmi metodick\u00fdmi p\u0159\u00edstupy, zejm\u00e9na s molekul\u00e1rn\u00edmi anal\u00fdzami.<\/p>\n<p>P\u0159\u00edstrojov\u00e9 vybaven\u00ed laborato\u0159e \u010d\u00edt\u00e1 pr\u016ftokov\u00fd cytometr Partec CyFlow Space (vybaven\u00fd UV-\u010dipem) vyu\u017e\u00edvan\u00fd zejm\u00e9na pro stanoven\u00ed stupn\u011b ploidie, pr\u016ftokov\u00e9 cytometry Partec CyFlow SL a Partec CyFlow Space (oba vybaven\u00e9 diodov\u00fdm laserem 532 nm) vyu\u017e\u00edvan\u00e9 zejm\u00e9na pro stanoven\u00ed absolutn\u00ed velikosti jadern\u00e9ho genomu, a p\u0159\u00edstroj CytoFlex (Beckman Coulter) se \u010dty\u0159mi sv\u011bteln\u00fdmi zdroji s variabiln\u00edm nastaven\u00edm (pro stanoven\u00ed ploidn\u00edho stupn\u011b i velikosti genomu), kter\u00e9 je v obou p\u0159\u00edpadech dopln\u011bn\u00e9 o bo\u010dn\u00ed (side scatter, SSC) i p\u0159\u00edm\u00fd (forward scatter, FSC) rozptyl. Pou\u017e\u00edv\u00e1 se zejm\u00e9na pro anal\u00fdzy s vysok\u00fdm rozli\u0161en\u00edm.<\/p>\n<p><strong>Kontakt:<\/strong><\/p>\n<ul>\n<li><a href=\"https:\/\/www.ibot.cas.cz\/cs\/lide\/detail-osoby\/?it=160\">Mgr. Ing. Pavel Tr\u00e1vn\u00ed\u010dek, Ph.D.<\/a><\/li>\n<li><a href=\"https:\/\/www.ibot.cas.cz\/cs\/lide\/detail-osoby\/?it=696\">RNDr. Zuzana Chumov\u00e1, Ph.D.<\/a><\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p><strong>Cen\u00edk z\u00e1kladn\u00edch anal\u00fdz pro extern\u00ed spolupracovn\u00edky\/projekty s P\u0159F UK:<br \/>\n<\/strong><\/p>\n<ul>\n<li>Stanoven\u00ed relativn\u00ed velikosti genomu (stupn\u011b ploidie) z \u010derstv\u00e9ho rostlinn\u00e9ho materi\u00e1lu vzhledem ke standardu se zn\u00e1mou ploidi\u00ed (s pou\u017eit\u00edm DAPI a UV lampy): 80,- K\u010d\/40,- K\u010d<\/li>\n<li>Stanoven\u00ed absolutn\u00ed velikosti jadern\u00e9ho genomu z \u010derstv\u00e9ho rostlinn\u00e9ho materi\u00e1lu (s pou\u017eit\u00edm propidium jodidu a laseru): 90,- K\u010d\/45,- K\u010d<\/li>\n<\/ul>\n<p>&nbsp;<\/p>\n<p>&nbsp;<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Laborato\u0159 pr\u016ftokov\u00e9 cytometrie byla zalo\u017eena v z\u00e1\u0159\u00ed 2000 a je nyn\u00ed sou\u010d\u00e1st\u00ed Odd\u011blen\u00ed evolu\u010dn\u00ed biologie rostlin. Zab\u00fdv\u00e1 se rychl\u00fdm stanoven\u00edm obsahu jadern\u00e9 DNA (velikosti genomu) v relativn\u00edch i absolutn\u00edch jednotk\u00e1ch. Z\u00edskan\u00e9 \u00fadaje jsou vyu\u017e\u00edv\u00e1ny ke studiu diverzity a dynamiky polyploidn\u00edch komplex\u016f c\u00e9vnat\u00fdch rostlin \u010di ke zhodnocen\u00ed evolu\u010dn\u00edch a ekologick\u00fdch d\u016fsledk\u016f genomov\u00e9 duplikace (polyploidie) a variability [&hellip;]<\/p>\n","protected":false},"author":15,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"_acf_changed":false,"footnotes":"","_links_to":"","_links_to_target":""},"class_list":["post-408","page","type-page","status-publish","hentry"],"acf":[],"_links":{"self":[{"href":"https:\/\/www.ibot.cas.cz\/oddeleni-evolucni-biologie-rostlin\/index.php?rest_route=\/wp\/v2\/pages\/408","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/www.ibot.cas.cz\/oddeleni-evolucni-biologie-rostlin\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/www.ibot.cas.cz\/oddeleni-evolucni-biologie-rostlin\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/www.ibot.cas.cz\/oddeleni-evolucni-biologie-rostlin\/index.php?rest_route=\/wp\/v2\/users\/15"}],"replies":[{"embeddable":true,"href":"https:\/\/www.ibot.cas.cz\/oddeleni-evolucni-biologie-rostlin\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=408"}],"version-history":[{"count":10,"href":"https:\/\/www.ibot.cas.cz\/oddeleni-evolucni-biologie-rostlin\/index.php?rest_route=\/wp\/v2\/pages\/408\/revisions"}],"predecessor-version":[{"id":768,"href":"https:\/\/www.ibot.cas.cz\/oddeleni-evolucni-biologie-rostlin\/index.php?rest_route=\/wp\/v2\/pages\/408\/revisions\/768"}],"wp:attachment":[{"href":"https:\/\/www.ibot.cas.cz\/oddeleni-evolucni-biologie-rostlin\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=408"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}