Inactivation of cytokinin-type phytohormones via N- and O-glucosylation – phylogeny and significance in evolution of hormonal homeostatic mechanisms

Name: Inactivation of cytokinin-type phytohormones via N- and O-glucosylation – phylogeny and significance in evolution of hormonal homeostatic mechanisms
Researchers: Záveská Drábková Lenka (co-researcher)
Project Type: B - national grants (GA ČR, GA AVČR)
Realization from: 2016
Realization to: 2018
Summary: The project addresses elucidation of phylogeny of N-glucosyltransferase pathway involved in irreversible inactivation of plant hormones cytokinins (CKs). The products of this pathway, CK N7- and N9-glucosides, are biosynthesized in plants by CK-N-glucosyltransferases encoded by two UGT genes, UGT76C1 and UGT76C2. In this way much broader spectrum of CKs can be inactivated than by metabolic degradation. Phylogenic and molecular analyses of UGT76C1 and UGT76C2 genes will be performed with the aim (1) to determine their distribution across the plant kingdom, (2) to unravel their evolution within the glucosyltransferase family using UGT76C1 and UGT76C2 as markers in addition to the known phylogenetic signals (18S rDNA, 26S rDNA, matK, atpB, rbcL etc.) and (3) to assess their significance in generation of mechanisms of hormonal homeostasis. In accordance with the proposed approaches (4) structural and functional manifestation of N-glucosyltransferase pathway will be characterized and (5) its biological significance within evolution of higher plants will be evaluated.

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